مطالعه تغییرات فعالیت‌های پاراکسونازی و آریل‌استرازی آنزیم پاراکسوناز به‌دنبال تجویز عصاره آبی سیر در موش صحرائی

  Received: 1 April, 2010 Accepted: 22 May, 2010   Abstract   Background & Aims : Paraoxonase 1 is one of the most significant enzymes scavenging of harmful free radicals and conserves blood lipoproteins against oxidant components. The present study aims to investigate the effects of aqueous garlic extract supplementation Paraoxonase 1.   Materials & Methods: Twenty normal rats which were divided randomly into two groups including 10 rats for each control and specimen groups. Specimen group received 3 ml of extract interperitoneally each day and the control group received normal saline in the same volume and condition during the experiment. After a week injection, routine blood analysis including Paraoxonase 1 , Arylesterase activity , and lipid parameters were performed. Oxidant malondialdehyde and antioxidant (total antioxidant status) parameters were also measured.   Results: Serum total cholesterol (p<0.05), low-density lipoprotein (p<0.05) and triglyceride (p<0.05) levels were found to be significantly lowered, but Paraoxonase 1 (p<0.05) , Arylesterase (p<0.05) , and HDL (p<0.05) level increased after using the extract. Blood total antioxidant status (p<0.05) values were found to have increased and malondialdehyde (p<0.05) level decreased during this episode. There was a positive and significant correlation between PON and Arylesterase (p=0.025, r=0.697). The correlation between Paraoxonase 1 and HDL-cholesterol concentration was also significant and positive (p=0.006, r=0.794).   Conclusion : It was concluded that aqueous garlic extract supplementation increases Paraoxonase 1 and Arylesterase improves blood lipid profile and strengthens blood antioxidant potential. It also leads to a decrease in the level of malondialdehyde in blood samples which demonstrates reduced oxidation reactions in the body.   Keywords: Aqueous garlic extract, Malondialdehyde, Paraoxonase, Arylesterase     Address: Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.   Tel: (+98) 9141145493     Email: [email protected]     Source: U rmia M ed J 2010: 21(2): 297 ISSN: 1027-3727